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• Clean the entire outside of the bag with isopropyl alcohol or any cleaners you are using, transportation could have left some unwanted contaminators and this ensures they're ready for you.      

• For syringes, heat the needle and inoculate the middle of the bag, use a clean piece of packing tape to seal the needle hole you just made OR cut a small corner of the top of the bag and inject liquid into the hole, seal however you wish.  Shake bag to distribute liquid culture.         

• For agar, using cutting tools, cut the bag seal at the top and open the bag in a front of a flow hood or clean air environment and transfer agar chunks into the bag. Seal or tie closed and shake to distribute the agar chunks.  

• For grain to grain transfers, follow the agar step and cut the bag open at the top seal. Add mycelium covered grains directly into the sterilized milo bag and seal or tie closed. Shake well to distribute mycelium thoroughly.     

   Finally, keep newly inoculated bags in a dark cool environment or however you wish!      

Not ready to use the bags yet?      

Store bags in a clean and cool space with little air flow and light. Try and use them within a month of receiving them.

Don’t worry about the smell, worry if you see pink, yellow or green colors in the substrate. Contact us right away! 

No need to add more moisture but feel free to add moisture while using it. 

Instructions: 

1 bag fits a 55-66qt tub or bin 

1 bag is 10lbs 

Take 3.5lbs - 4lbs of mycelium (grain spawn) and mix thoroughly with substrate.

Save a few handfuls of substrate for a top layer, use this amount after you make the cake and cover the exposed mycelium evenly with this extra substrate. 

Storage: Shelf life is about 2-3 months. Keep in cool room or basement. 

For long term storage you can freeze the blocks

Need a shopping list of monotub supplies? Shegrowsfungi.com

Instructions: 

Mixing up the Grain Spawn and Substrate in a Mono Tub

1 Poo in a Tub Grow Kit bag fits a 54-66qt tub 

1. Clean: Spray the colonized bag and substrate bag with isopropyl alcohol. Spray liner, mono tub, and lid with isopropyl and wipe with a paper towel. 

2. Open: Cut that 4lb of colonized grains and substrate bag open. 

3. Mix: Pour both the healthy colonized grains andsubstrate into the mono tub and mix thoroughly.  Saving a few handfuls of substrate for the casing layer step.

4. Shaping: Gently pat the mixed spawn and substrate to make a nice cake. 

5. Casing Layer: Save few handfuls of substrate for a top layer, use this amount after you make the cake and cover the exposed mycelium evenly with this extra substrate. 

6. Lastly: Water or heavily mist the cake and tub for added moisture.

7. Wait! Now is the best part. The mycelium will slowly start to overtake and grow throughout the cake. Soon mushrooms will pop up to harvest.

8. Harvest and rehydrating: We suggest “twist and pull” all the mushrooms out of the cake and rehydrate with a heavy mist of clean water. Close the lid and let them recolonize to grow another flush. 

Instructions:

1. All Poo in a Bag Kits include, 1 colonized grain bag, 4 x 3lb substrate bags in XLA style breathable bags and 4 x office clips.

2. Inspect all items for contamination and wipe items down with isopropyl.

3. Prepare workspace.

4. Shake colonized grain bag for inoculation. 

5. Open all four substrate bags for easy transfers.

6. Using clean scissors cut open grain bag and pour an even amount of grain spawn into each of the four substrate bags. 

8. Shake bags well and get those grains distributed evenly. 

9. Pat cake down evenly to form a block inside the bag.

10. Using the office clips, roll the top of the bag down to the filter and clip each bag. 

11.  Label and date all the bags.

12.  For fruiting conditions, keep temperatures below 75 degrees and allow 10 hours of light. 

13.  For harvesting, using clean gloves, open a bag and twist and pull each mushroom out and cut off any extra substrate.

14.  For the second flush after the first harvest, spray some clean water into the bag and onto the cake. Close and clip and wait for the next round. 

15.  Video instructions are available on PooGodco.com under the “How to Use” tab.

For any more questions just message us directly! Enjoy 

Instructions: 

Inoculating the Grain Bag with the Syringe

1. Clean:  The entire outside of the grain bag with isopropyl alcohol or any cleaners you are using, transportation could have left some unwanted contaminators, and this ensures they're ready for you. 

2. Inoculate the grain bag: Open the syringe box and take out the blue bag. Remove the liquid culture and needle from sterile bags. Attach the needle to syringe and heat the needle until red hot, let cool and poke the injection port on the bag and inject liquid (5-10 ml/cc) into the hole. Some people put a piece of clean tape on that hole. Shake bag to distribute liquid culture. 

3. Wait: The bag will turn white with mycelium after a few weeks. Feel free to shake the bag and distribute mycelium for faster colonization.  Check to make sure it’s clean and healthy by appearance. (Any sour or rotten smell is contamination)

4. Prepare for mono tub stage:Gather the materials needed for a mono tub. Need a shopping list of monotub supplies? Shegrowsfungi.com (Front page has a PDF with pictures of items needed)

Instructions: 

Mixing up the Grain Spawn and Substrate in a Mono Tub

1 grow kit bag fits a 54-66qt tub 

1. Clean: Spray the colonized bag and substrate bag with isopropyl alcohol. Spray liner, mono tub, and lid with isopropyl and wipe with a paper towel. 

2. Open: Cut that 4lb of colonized grains and substrate bag open. 

3. Mix: Pour both the healthy colonized grains and ¾ substrate into the mono tub and mix thoroughly. 

4. Shaping: Gently pat the mixed spawn and substrate to make a nice cake. 

5. Casing Layer:Use that last ¼ substrate and add a top pseudo soil layer, pat down also. 

6. Lastly: Water or heavily mist the cake and tub for added moisture.

7. Wait! Now is the best part. The mycelium will slowly start to overtake and grow throughout the cake. Soon mushrooms will pop up to harvest.

8. Harvest and rehydrating: We suggest “twist and pull” all the mushrooms out of the cake and rehydrate with a heavy mist of clean water. Close the lid and let them recolonize to grow another flush.